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- Undertake phylogenetic
analyses of ß-glucosidase and ß-galactosidase gene families
- Obtain cDNAs encoding
complete coding regions
- Overexpress the
hydrolases in active and soluble form in appropriate expression
systems
- Purify recombinant
hydrolases and identify their biological roles by determining
their aglycone specificities
- Determine temporal and
spatial expression patterns of target hydrolases
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