Steven Green - Department of Biology and Department of Otolaryngology - Head and Neck Surgery
Marlan Hansen - Department of Otolaryngology - Head and Neck Surgery
The Tissue/Cell Culture Core (TC core) provides facilities and expertise for the development and
preparation of in vitro models for study of cochlear development, function, and consequences of deafening or trauma.
Assists investigators in producing cultures for use in their research on the cochlea.
Assists investigators in genetic manipulation of cultured cochlear cells and tissues using transfection, virus-mediated gene transfer, and the use of genetically modified animals.
Assists investigators in developing and optimizing new in vitro model systems.
Trains investigators, students, postdocs, residents, and staff in culture techniques and assists in troubleshooting problems.
Coordinates multidisciplinary experiments among investigators and the Genomics and Histology & Imaging cores that use live cultures prepared in the TC core.
Promotes and supports collaborations among investigators to use multidisciplinary approaches with in vitro models or parallel in vivo and in vitro models.
Individual experiments may use multiple cores. Each of the cores was established to provide the
greatest interaction between ICMAN faculty and to provide an opportunity to further potential collaborations.
There are two comparably-equipped facilities, one in 5256D CBRB managed by Jason Clark, the other in
232 BB managed by Cathy Kane. Both are fully equipped for most culture techniques, with biosafety cabinets (sterile hoods), CO2
incubators, dissection stations, centrifuges, and inverted microscopes. Investigators can use the facilities to dissect rodent
tissues and prepare and maintain primary cultures or maintain cell lines.
For the most experienced users who may not have fully-equipped tissue culture facilities, the core will
provide just that: a facility in which they can schedule time to do dissections, prepare and maintain cultures, including
routine observation of the health of the culture using phase contrast microscopy.
Less experienced users will be able to obtain advice and assistance in preparing and maintaining
cultures. This includes teaching and observation of culture technique to improve performance and troubleshoot problems. To
facilitate training, the core has available video-equipped dissecting microscopes and a video library of demonstrations of
dissection and culture techniques.
Least experienced users will be served by having the TC core RA prepare and maintain cultures for
their experiments while being trained in culture techniques.
The core directors and RAs will also work with investigators to develop or modify culture
preparations to optimize them for particular experiments.
The Tissue/Cell Culture Core can provide limited supplies for use by investigators. Please check the Supplies List for more information.
Schedule a Consultation
If you require consultation or training on a project for the Tissue/Cell Culture Core, please contact
the following to schedule an appointment: